Application Notes
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AN002: Multi-compound electrophoretic assays for biocatalytic activity with the ActiPix™ D100 (pdf, 319kb)
This application note describes how the ActiPix™ UV area imaging detector can be used to test biocatalyst substrate specificity towards a mixture of UV active compounds using a continuous engagement electrophoretically mediated microanalysis (EMMA) assay method.
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AN001: Measurement of hydrodynamic radius for a standard protein over a wide concentration range (pdf, 0.66MB)
This application note shows use of the ActiPix™ for determination of hydrodynamic radius of a protein. The method allows proteins to be used in their native form, without any labelling or denaturation. A plug of protein solution is injected into a fused silica capillary, driven through the capillary by application of pressure, and detected using UV area imaging as it passes windows at entrance to and exit from a loop in the capillary. The radius of the protein is determined by analysis of band broadening due to Taylor dispersion. The method is applicable over a wide concentration range and uses only nanolitres of sample.