Applications Library
The following documents are demonstrations of real time applications using this product. You must register to download these.
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ActiPix™ Brochure (pdf, 1.01MB)
Eight page PDF brochure introducing the ActiPix™ D100 UV Imaging Detector.
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AN014: On column detection monolithic fused silica capillary (pdf, 0.68MB)
This application note illustrates on-column detection using a monolithic capillary column 100 mm i.d./ 360 mm o.d. and isocratic elution. Flow resistance in monolithic columns is much lower than in conventional particle packed columns, and a syringe pump is used to drive the flow. For 1 pmol loading of caffeine and 0.5 s time constant, the signal-to-noise ratio is 23. This shows that the ActiPix™ D100 detector has femtomole sensitivity for on-column detection. A particular benefit of imaging through the column bed with the ActiPix™ D100 is that time-displaced averaging used in Paraytec’s proprietary software removes any random fluctuations in the signal due to non-uniformity of the stationary phase and scattering effects. As shown with a sharply fronting peak of caffeine under overload conditions, this occurs without any sacrifice of the 70 mm spatial resolution.
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AN013: Analysis of peptides in nanoLC (pdf, 0.92MB)
This application note illustrates use of the ActiPix™ D100 for detection of three peptides (angiotensin II, Met-enkephalin and Leu-enkephalin) following separation by nanoLC on a 75 mm i.d. column. Using a nanoLC cartridge with detection capillary having i.d. 75 mm, results are shown for loadings of 0.5 ng each peptide. The S/N for Leu-enkephalin is 63, suggesting that the limit of detection is below 0.05 ng (80 fmol). Detection is also demonstrated in the 20 mm i.d. fused silica transfer line, and with loading of 1 ng each peptide on column the S/N for Leu-enkephalin is 4.4. Peak broadening due to Taylor dispersion in the wider bore capillary causes the peaks to be slightly wider than those measured directly in the transfer line.
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AN003: Substrate specificity screening with UV area imaging detector (pdf, 0.86MB)
This application note describes how the ActiPix™ UV area imaging detector can be used to test penicillinase (β-lactamase) active towards a mixture of UV active substrates using an electrophoretically mediated microanalysis (EMMA) assay method.1 A variety of bacteria produce the β-lactamase enzymes which catalyse the ring-opening of β-lactam-based antibiotics. Identifying those compounds that are resistant to this process is important as it is responsible for bacterial resistance to penicillin and other β-lactam antibiotics.
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AN004: lab-on-capillary systems (pdf, 0.87MB)
This application note describes how the ActiPix™ UV area detector can be used in a number of lab-on-capillary setups that combine various reaction, separation and detection steps. By assembling integrated systems from readily available components nanoscale reactions can be carried out that minimise the consumption of expensive materials.
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TN002: connecting to Agilent HP3DCE (pdf, 0.82MB)
Directions for connecting to Agilent HP3DCE
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TN003: Comparison of TSU and TSP capillaries (pdf, 0.76MB)
Advantages and disadvantages of TSU coated capillaries
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TN004: Area Imaging Sensors (pdf, 0.99MB)
The advantages and benefits of area imaging for UV detection
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TN005: Installing Capillary Window in a nanoLC Cartridge (pdf, 0.75MB)
Directions for Installing a Capillary Window in a nanoLC Cartridge
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TN007: How to mount the ActiPix™ Sensor head (pdf, 395kb)
With its small size the D100 is easy to locate areas previously unavailable for UV detectors. Here is a simple guide for mounting securely for any application.
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TN008: Sample velocity calculation (pdf, 306kb)
How the ActiPIx software calculates peak velocities for the purpose of data normalisation across an area