The following documents are demonstrations of real time applications using this product. You must register to download these.
An early article from the June 2008 edition of Chromatography Today discussing the principles behind Taylor dispersion analysis.
The ActiPix™ TDA200 is a benchtop high throughput nano-sizing system which enables the rapid determination of size and purity for a range of molecules including proteins, antibodies, nanoparticles and quantum dots. It measures the hydrodynamic radius and diffusion coefficient for species in solution.
This application note shows use of the ActiPix™ for determination of hydrodynamic radius of a protein. The method allows proteins to be used in their native form, without any labelling or denaturation. A plug of protein solution is injected into a fused silica capillary, driven through the capillary by application of pressure, and detected using UV area imaging as it passes windows at entrance to and exit from a loop in the capillary. The radius of the protein is determined by analysis of band broadening due to Taylor dispersion. The method is applicable over a wide concentration range and uses only nanolitres of sample.
Quantum dots are typically fluorescent nanoparticles of high quantum efficiency (see Figure 1). They can be custom synthesized for a wide variety of applications ranging from medical imaging to next generation LCD displays. Paraytec have developed a new approach to determining the hydrodynamic radius of species in solution. This application note is a proof of principle study to demonstrate the use of the ActiPix™ HT Nano-Sizing System for determination of hydrodynamic radii of quantum dot and gold nanoparticle samples provided by the Physics Department at the University of Leeds. Excellent correlation was obtained between experimental and expected values with typical analysis times of less than 10 minutes. This approach is a significantly faster and more cost efficient approach compared to transmission electron microscopy (TEM) which typically takes several hours to perform this analysis. In addition, conventional particle measuring techniques cannot effectively measure down to sub 20 nanometre sizes.
Formula for calculating Taylor Dispersion
Use of the ActiPix™ UV area imaging detector with multiple windows on a single looped capillary provides new insights with species of pharmaceutical & biopharmaceutical interest. Two key applications are demonstrated: Determination of hydrodynamic radius from imaging pressure driven flow of analyte bands at two windows. Results are shown for proteins and small molecules injected as single species and in mixtures, and for a mixture of proteins with simultaneous electrophoretic separation. Determining substrate specificity of a biocatalyst towards a mixture of UV active compounds using a continuous engagement electrophoretically mediated microanalysis (EMMA) method.
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